Selected Sequence features of Universal Proteomics Resources
loxP:
ATAACTTCGTATAGCATACATTATACGAAGTTAT
Splice Donor (SD) and 6xHN epitope tag from pDNR-Dual vector:
GTAAGTGGTCATAATCATAATCATAATCATAATCATAATCACAAC
Splice Donor (SD):
GTAAGT
Note: the splice occurs at the base immediately preceeding the GTAAGT.
6xHN epitope tag:
CATAATCATAATCATAATCATAATCATAATCACAAC
Splice Acceptor (SA):
TCCCTTTTTTTTCCACAG
Note: the splice occurs at the base immediately following the ACAG.
Forward PCR-cloning primer (for In-Fusion PCR cloning into pDNR-Dual vector)
5'-GAAGTTATCAGTCGAC[gene-specific-sequence]-3'
Reverse PCR-cloning primer (for In-Fusion PCR cloning into pDNR-Dual vector)
5'-ATGGTCTAGAAAGCTT[gene-specific-sequence]-3'