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Two types of P1 vectors The P1 mapping library consists of clones generated in two related P1 cloning vectors. 3,840 clones were inserted into the vector known as pNS583tet14Ad10 (Smoller et al. 1991) and 5,376 clones were from the ligation mixture using the vector called pSacBII (Kimmerly et al. 1996). Both vectors contain genes that allow resistance to the antibiotic kanamycin, a plasmid origin of replication that provides for a single copy, a loxP site (the cis-acting site specific recognition signal for the P1 recombinase), and a gene that allows resistance to tetracycline. Inserts cloned in the vector pNS583tet14Ad10 have been introduced into the BamHI site in the gene allowing tetracycline resistance. A sacBII cassette was introduced by Sternberg (1990) as a means of selecting for clones with inserts. The cloned inserts in this vector system are introduced between SP6 and T7 RNA polymerase promoters that are positioned of the Bacillus subtilis genes found in the SacBII cassette. The sequences between the loxP sites (containing the Ad10 sequences) are deleted during library construction and thus are not present when the clones are mapped and eventually sequenced. At the bottom of this page is the sequence for the two vectors in fasta format. Map of pNS583tet14Ad10 from FlyBase (note: this vector is actually 14574bp long) Map of pSacBII (note: this vector is actually 16005bp, not 16004bp) 
Kimmerly, W., et al. (1996) A P1-based physical map of the Drosophila euchromatic genome. Genome Research 6: 414-430. Smoller, D.A., Petrov, D. and Hartl, D.L. (1991) Characterization of bacteriophage P1 library containing inserts of Drosophila DNA of 75-100 kilobase pairs. Chromosoma 100: 487-494. Sternberg, N. (1990) Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs. Proc. Natl. Acad. Sci. USA 87: 103-107. If you have questions, please e-mail bdgp@fruitfly.berkeley.edu. >pNS582tet14Ad10 14574bp GATCCACAGGACGGGTGTGGTCGCCATGATCGCGTAGTCGATAGTGGCTC CAAGTAGCGAAGCGAGCAGGACTGGGCGGCGGCCAAAGCGGTCGGACAGT GCTCCGAGAACGGGTGCGCATAGAAATTGCATCAACGCATATAGCGCTAG CAGCACGCCATAGTGACTGGCGATGCTGTCGGAATGGACGATATCCCGCA AGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGCATCCAGG GTGACGGTGCCGAGGATGACGATGAGCGCATTGTTAGATTTCATACACGG TGCCTGACTGCGTTAGCAATTTAACTGTGATAAACTACCGCATTAAAGCT TATCGATGATAAGCTGTCAAACATGAGAATTGATCCGGAACCCTTAATAT AACTTCGTATAATGTATGCTATACGAAGTTATTAGGTCCCTCGACTACGT CGTTAAGGCCGTTTCTGACAGAGTAAAATTCTTGAGGGAACTTTCACCAT TATGGGAAATGGTTCAAGAAGGTATTGACTTAAACTCCATCAAATGGTCA GGTCATTGAGTGTTTTTTATTTGTTGTATTTTTTTTTTTAGAGAAAATCC TCCAATATATAAATTAGGAATCATAGTTTCATGATTTTCTGTTACACCTA ACTTTTTGTGTGGTGCCCTCCTCCTTGTCAATATTAATGTTAAAGTGCAA TTCTTTTTCCTTATCACGTTGAGCCATTAGTATCAATTTGCTTACCTGTA TTCCTTTACATCCTCCTTTTTCTCCTTCTTGATAAATGTATGTAGATTGC GTATATAGTTTCGTCTACCCTATGAACATATTCCATTTTGTAATTTCGTG TCGTTTCTATTATGAATTTCATTTATAAAGTTTATGTACAAATATCATAA AAAAAGAGAATCTTTTTAAGCAAGGATTTTCTTAACTTCTTCGGCGACAG CATCACCGACTTCGGTGGTACTGTTGGAACCACCTAAATCACCAGTTCTG ATACCTGCATCCAAAACCTTTTTAACTGCATCTTCAATGGCCTTACCTTC TTCAGGCAAGTTCAATGACAATTTCAACATCATTGCAGCAGACAAGATAG TGGCGATAGGGTTGACCTTATTCTTTGGCAAATCTGGAGCAGAACCGTGG CATGGTTCGTACAAACCAAATGCGGTGTTCTTGTCTGGCAAAGAGGCCAA GGACGCAGATGGCAACAAACCCAAGGAACCTGGGATAACGGAGGCTTCAT CGGAGATGATATCACCAAACATGTTGCTGGTGATTATAATACCATTTAGG TGGGTTGGGTTCTTAACTAGGATCATGGCGGCAGAATCAATCAATTGATG TTGAACCTTCAATGTAGGGAATTCGTTCTTGATGGTTTCCTCCACAGTTT TTCTCCATAATCTTGAAGAGGCCAAAACATTAGCTTTATCCAAGGACCAA ATAGGCAATGGTGGCTCATGTTGTAGGGCCATGAAAGCGGCCATTCTTGT GATTCTTTGCACTTCTGGAACGGTGTATTGTTCACTATCCCAAGCGACAC CATCACCATCGTCTTCCTTTCTCTTACCAAAGTAAATACCTCCCACTAAT TCTCTGACAACAACGAAGTCAGTACCTTTAGCAAATTGTGGCTTGATTGG AGATAAGTCTAAAAGAGAGTCGGATGCAAAGTTACATGGTCTTAAGTTGG CGTACAATTGAAGTTCTTTACGGATTTTTAGTAAACCTTGTTCAGGTCTA ACACTACCGGTACCGCGCTTGCGGAAGCATCAGCAAATAAGGCCAGCACA GCCAGCGCAGTTGCCGCTTTGGTTCCTGATTCTGTTCTTGATGAATTAAA CAAAGCGGCACAGTAACAAAGGACTTCATTGATAATTTTTCTTCAGGAGG AAGACATGTCATTCTTTTCTACGTTAAAAACAGCTTTGTCTTTGAAGGAG AAACTTGCTGCTACTGGTGTTCTTGTTCTGATTTGCGCACTTGTTGGTGC TGGGTTTGCATGGGAACGTCATCAGCTAAAGCAAGCCATAGAGAAAATTG GCAGTCTTGATCAGGCTGTTAAGGAACGTGATAAGTCAATAATGGATCTT AACCAGACCATTGAGACGATGAACAAAGCAGAGCAACATTTTCACAGCCA GGAAGTGAAAAATGAATCAGAACAAGCCAAGTATGCTGACAGGCAAATGG AACGAAAAGCTGAAGTTCAGAAACAACTGGTTGCGGCGGGTAATGTTCGC CAGCGTATTCCTGCTGACACTCAGCGGTTGCTCCGGGAGTCGATCAGCGA ATTTAACGCCGACGCCGACAAAGGTTAACCACCCTGCCCCCAAAAGTGCA TTTATGTGTAGGATGCCAGAGTTTAGCAGTGAATATTTTGATGATCTGCC AGCGTATATCCTCGATACAGAAACGATGCTGATGGGGATTAACAGGAAGA ATCGCAACGTTAATGATTACAACCGAGCTATTAGCGGTAACTAAAAGGGA TTTTTATGTCTGATAAAGTAACAGTAAAGCAAACTATCAACAAAGCGACT TCAATCTACAAAATTGAGCAAATCACTGTTGGCAAGCCAGGATCTGAACA ATACCGTCGTGCTTTCGAGCTTGCCGATCAGCTTGGTTTAAAACACCCGG ATTGCATTGAGCATGTATTTCCGACCTATGCTGATGAGCAATGTACTCAT GTTCTTACCGAAGAGGATTTTTTCAGCACTGAAGAACGAGAAGGCGTTGA TCGCTGCATTGGTGTGATTTGTTCTTCGGTAAGTGATGAGTTATTCCCTA ATGTGCCTGAATATGGTGGTATTGGATACCAATTCCTGTACGAGGGCGAT GAGCTTAAATGCTATGAACATGGTCTTCTCATCGAAAGCGTAGAATAATA CGACTCCCTTCCAACCGGCTACGTTGGCCGGTTTTTCACTTATCCACATT ATCCACTGGATAGATCCAATAATCAGGTCCATACAGATCCCAATTAGATC CATATAGATCCCTGATCGTTGCAGGCCGCGCCACGTCTGGCTTAGAAGTG TATCGCGATGTGTGCTGGAGGGAAAACGATGTGTGCTGGAGGGATAAAAA TGTGTGCTGACGGGTTGCTAATGTGTGCTGGCGGGATATAGGATGTGTGT TGACGGGAAAGCTTGGGTAGTTATCACCACTTATAAAAACTATCCACACA ATTCGGAAAAAGTAATATGAATCAATCATTTATCTCCGATATTCTTTACG CAGACATTGAAAGTAAGGCAAAAGAACTAACAGTTAATTCAAACAACACT GTGCAGCCTGTAGCGTTGATGCGCTTGGGGGTATTCGTGCCGAAGCCATC AAAGAGCAAAGGAGAAAGTAAAGAGATTGATGCCACCAAAGCGTTTTCCC AGCTGGAGATAGCTAAAGCCGAGGGTTACGATGATATTAAAATCACCGGT CCTCGACTCGATATGGATACTGATTTCAAAACGTGGATCGGTGTCATCTA CGCGTTCAGCAAATACGGCTTGTCCTCAAACACCATCCAGTTATCGTTTC AGGAATTCGCTAAAGCCTGTGGTTTCCCCTCAAAACGTCTGGATGCGAAA CTGCGTTTAACCATTCATGAATCACTTGGACGCTTGCGTAACAAGGGTAT CGCTTTTAAGCGCGGAAAAGATGCTAAAGGCGGCTATCAGACTGGTCTGC TGAAGGTCGGGCGTTTTGATGCTGACCTTGATCTGATAGAGCTGGAGGCT GATTCGAAGTTGTGGGAGCTGTTCCAGCTTGATTATCGCGTTCTGTTGCA ACACCACGCCTTGCGTGCCCTTCCGAAGAAAGAAGCTGCACAAGCCATTT ACACTTTCATCGAAAGCCTTCCGCAGAACCCGTTGCCGCTATCGTTCGCG CGAATCCGTGAGCGCCTGGCTTTGCAGTCAGCTGTTGGCGAGCAAAACCG TATCATTAAGAAAGCGATAGAACAGCTTAAAACAATCGGCTATCTCGACT GTTCTATTGAGAAGAAAGGCCGGGAAAGTTTTGTAATCGTCCATTCTCGC AATCCAAAGCTGAAACTCCCCGAATAAGTGTGTGCTGGAGGGAAACCGCA TTAAAAAGATGTGTGCTGCCGGGAAGGCTTGTCCAATTTCCTGTTTTTGA TGTGCGCTGTAGTGGACAGCCCGTCAGTTTGCCCAGACTTTCCCTCCAGC ACACATCTGTCCATCCGCTTTTCCCTCCAGTGCACATGTAATTCTCTGCC TTTCCCTCCAGCACACATATTTGATACCAGCGATCCCTCCACAGCACATA ATTCAATGCGACTTCCCTCTATCGCACATCTTAGACTTTTATTCTCCCTC CAGCACACATCGAAGCTGCCGGGCAAGCCGTTCTCACCAGTTGATAGAGA GTGAAGCTTGGCTGCCCATTGAAGCAGGAAATCACCAAAATGATTCAGGC TACAACCTGAACGTAGAAGAAATCCGCGTCCTTTATGCGTGGAGGATGCC AAAGCATGTTGTGACACACTTGGCAAAGGAGTAAGCATGCAGAGAATGCT ATGTACAAGCATCTACGCATACATTATTATTTTATGCAGCATTTTTAATT AAATTCAAAAATACAGCATAAAGGATGACTTTCGATGAGTGATTCCAGCC AGCTTCACAAGGTTGCTCAAAGAGCAAACAGAATGCTCAATGTTCTGACT GAACAAGTACAGTTGCAAAAGGATGAGCTACACGCGAACGAGTTTTACCA GGTCTATGCGAAAGCGGCACTGGCAAAATTGCCTCTACTGACTCGAGCGA ACGTTGACTATGCCGTAAGTGAAATGGAAGAAAAGGGTTATGTTTTCGAT AAACGCCCTGCTGGCTCTTCAATGAAATATGCGATGTCAATTCAGAACAT CATTGACATATATGAACATCGCGGAGTGCCAAAATACCGGGATCGCTACA GCGAAGCGTATGTGATTTTCATCTCCAATCTTAAAGGCGGTGTGTCAAAA ACTGTATCGACGGTTTCTCTGGCGCATGCAATGCGTGCTCACCCTCATCT TCTTATGGAGGATTTAAGGATTCTGGTTATTGACCTTGATCCGCAATCTT CAGCAACGATGTTTTTAAGCCATAAACACTCTATTGGTATCGTAAACGCA ACATCTGCACAGGCTATGTTGCAGAATGTAAGCCGTGAAGAGCTGTTAGA GGAGTTTATTGTTCCTTCTGTTGTACCTGGGGTTGACGTTATGCCTGCGT CGATTGACGATGCCTTTATTGCATCCGATTGGAGAGAGCTGTGCAATGAG CATCTACCGGGTCAGAACATCCATGCTGTCCTGAAAGAAAATGTGATTGA TAAGCTGAAGAGCGATTATGACTTTATCCTCGTTGATAGTGGTCCTCACC TTGACGCCTTCCTGAAAAATGCTTTGGCCTCGGCCAATATACTGTTTACA CCTCTGCCGCCAGCAACTGTCGATTTCCACTCATCGCTTAAATACGTTGC CCGCCTTCCTGAGTTGGTGAAACTCATTTCGGATGAAGGCTGCGAGTGCC AGCTTGCGACTAACATTGGTTTTATGTCCAAGTTGAGTAACAAGGCAGAC CATAAGTATTGCCATAGCCTGGCTAAAGAAGTGTTCGGTGGGGATATGCT TGATGTCTTCCTCCCTCGCCTTGACGGTTTTGAACGCTGCGGCGAGTCTT TTGACACTGTTATTTCAGCTAACCCGGCAACGTATGTTGGTAGTGCTGAT GCATTGAAGAACGCGCGAATTGCCGCGGAAGATTTTGCTAAAGCAGTTTT TGACCGTATTGAATTTATCAGATCTAACTGAGGAGTAAGAAACCCCCATG TCAAAGAAAAACAGACCAACAATTGGGCGAACCCTTAATCCTTCAATATT AAGCGGATTTGATAGTTCTTCAGCCTCTGGCGATCGAGTCGAGCAGGTAT TCAAGTTATCAACTGGTCGCCAGGCCACATTTATTGAAGAGGTAATACCT CCGAACCAGGTAGAAAGCGATACCTTTGTTGATCAGCATAACAACGGGCG TGACCAGGCATCTCTTACGCCAAAATCATTAAAAAGTATCCGAAGCACTA TTAAGCATCAGCAATTTTACCCTGCAATAGGTGTTAGACGGGCTACAGGG AAAATTGAAATTTTGGATGGTTCCCGGCGTCGAGCTTCTGCCATCTTAGA GAACGTAGGGTTGCGGGTTTTAGTCACGGACCAGGAGATCAGCGTTCAGG AAGCGCAAAATTTAGCGAAAGACGTTCAGACAGCATTGCAGCACAGCATT CGAGAAATAGGTCTGCGTTTGATGCGAATGAAAAATGATGGGATGAGTCA GAAGGATATTGCAGCCAAAGAAGGGCTGTCTCAGGCGAAGGTCACGCGTG CTCTCCAGGCAGCGAGTGCTCCGGAAGAATTAGTCGCCCTTTTCCCTGTG CAGTCGGAATTAACCTTTTCGGACTACAAAACGCTTTGTGCTGTTGGCGA CGAAATGGGGAACAAGAATTTAGAGTTTGATCAGCTTATTCAAAACATAT CCCCGGAAATAAGCGACATCTTATCCATTGAAGAAATGGCAGAAGATGAA GTTAAAAATAAAATCGTGCGCTTGATAACAAAGGAAGCCTCACTACTCAC GGATAAAGGTTCTAAAGATAAGTCCGTAGTTACTGAATTATGGAAATTTG AGGACAAGGATCGCTTTGCAAGGAAGCGCGTGAAAGGCCGTGCATTTTCT TATGAGTTTAATCGACTCTCAAAAGAGTTACAGGAAGAACTCGACAGGAT GATTGGGCATATCCTTAGAAAGAGCCTCGATAAAAAGCCGAAGCCTTAAA CTTTCGCCATTCAAATTTCACTATTAACTGACTGTTTTTAAAGTAAATTA CTCTAAAATTTCAAGGTGAAATCGCCACGATTTCACCTTGGATTTTACCT TCCTCCCCTCCTCCCGAAAAAAATAAAAAAATTGCTTGTCACGAGAAAGT CAACAAGTGACTTTCAATAAAATCTCTTCCGAAAAGGGATTCACACAAGT GCCTTGTGTTTAAGGAAGAGTAAATTGAGTAACTTACGCGAATACCAGAA TCGTATTGCAGATATCGCAAAACGCTCTAAAGCTGTGCTTGGCTGGGCAA GCACTGCGCAGTTCGGTACTGATAACCAATTCATTAAAGATGATGCCGCG CGTGCCGCATCTATCCTTGAAGCTGCACGTAAAGACCCGGTTTTTGCGGG TATCTCTGATAATGCCACCGCTCAAATCGCTACAGCGTGGGCAAGTGCAC TGGCTGACTACGCCGCAGCACATAAATCTATGCCGCGTCCGGAAATTCTG GCCTCCTGCCACCAGACGCTGGAAAACTGCCTGATAGAGTCCACCCGCAA TAGCATGGATGCCACTAATAAAGCGATGCTGGAATCTGTCGCAGCAGAGA TGATGAGCGTTTCTGACGGTGTTATGCGTCTGCCTTTATTCCTCGCGATG ATCCTGCCTGTTCAGTTGGGGGCAGCTACCGCTGATGCGTGTACCTTCAT TCCGGTTACGCGTGACCAGTCCGACATCTATGAAGTCTTTAACGTGGCAG GTTCATCTTTTGGTTCTTATGCTGCTGGTGATGTTCTGGACATGCAATCC GTCGGTGTGTACAGCCAGTTACGTCGCCGCTATGTGCTGGTGGCAAGCTC CGATGGCACCAGCAAAACCGCAACCTTCAAGATGGAAGACTTCGAAGGCC AGAATGTACCAATCCGAAAAGGTCGCACTAACATCTACGTTAACCGTATT AAGTCTGTTGTTGATAACGGTTCCGGCAGCCTACTTCACTCGTTTACTAA TGCTGCTGGTGAGCAAATCACTGTTACCTGCTCTCTGAACTACAACATTG GTCAGATTGCCCTGTCGTTCTCCAAAGCGCCGGATAAAGGCACTGAGATC GCAATTGAGACGGAAATCAATATTGAAGCCGCTCCTGAGCTGATCCCGCT GATCAACCACGAAATGAAGAAATACACCCTGTTCCCAAGTCAGTTCGTTA TCGCGGCTGAGCACACGGTACAGGCGGCGTATGAAGCACAGCGTGAATTT GGTCTGGACCTGGGTTCCCTACAGTTCCGCACCCTGAAGGAATACCTGTC TCATGAACAGGATATGCTGCGTCTTCGCATCATGATCTGGCGCACTCTTG CGACCGACACCTTTGACATCGCTCTGCCGGTTAACCAGTCCTTTGATGTA TGGGCAACCATCATTCGTGGCAAATTCCAGACTGTATATCGCGACATTAT TGAGCGCGTTAAATCTTCTGGTGCGATGGGGATGTTTGCTGGTGCTGATG CAGCATCTTTCTTCAAACAGTTGCCGAAGGATTTCTTCCAGCCAGCCGAA GACTATATCCAGACTCCGTATGTTCACTACATCGGTACCCCATTTAGGAC CACCCACAGCACCTAACAAAACGGCATCAGCCTTCTTGGAGGCTTCCAGC GCCTCATCTGGAAGTGGAACACCTGTAGCATCGACCTGCAGGGGGGGGGG GGCGCTGAGGTCTGCCTCGTGAAGAAGGTGTTGCTGACTCATACCAGGCC TGAATCGCCCCATCATCCAGCCAGAAAGTGAGGGAGCCACGGTTGATGAG AGCTTTGTTGTAGGTGGACCAGTTGGTGATTTTGAACTTTTGCTTTGCCA CGGAACGGTCTGCGTTGTCGGGAAGATGCGTGATCTGATCCTTCAACTCA GCAAAAGTTCGATTTATTCAACAAAGCCGCCGTCCCGTCAAGTCAGCGTA ATGCTCTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTC ATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATAC CATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGG CAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCG TCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTAT CAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAA AGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTC GTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCG CCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACA GGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATT TTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGG GGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGC TTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCAT CTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACA ACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGAT TGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCAT GTTGGAATTTAATCGCGGCCTCGAGCAAGACGTTTCCCGTTGAATATGGC TCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTT CATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGA CACAACGTGGCTTTCCCCCCCCCCCCTGCAGGTCGATAGCAGCACCACCA ATTAAATGATTTTCGAAATCGAACTTGACATTGGAACGAACATCAGAAAT AGCTTTAAGAACCTTAATGGCTTCGGCTGTGATTTCTTGACCAACGTGGT CACCTGGCAAAACGACGATCTTCTTAGGGGCAGACATTAGAATGGTATAT CCTTGAAATATATATATATATTGCTGAAATGTAAAAGGTAAGAAAAGTTA GAAAGTAAGACGATTGCTAACCACCTATTGGAAAAAACAATAGGTCCTTA AATAATATTGTCAACTTCAAGTATTGTGATGCAAGCATTTAGTCATGAAC GCTTCTCTATTCTATATGAAAAGCCGGTTCCGGCGCTCTCACCTTTCCTT TTTCTCCCAATTTTTCAGTTGAAAAAGGTATATGCGTCAGGCGACCTCTG AAATTAACAAAAAATTTCCAGTCATCGAATTTGATTCTGTGCGATAGCGC CCCTGTGTGTTCTCGTTATGTTGAGGAAAAAAATAATGGTTGCTAAGAGA TTCGAACTCTTGCATCTTACGATACCTGAGTATTCCCACAGTTAACTGCG GTCAAGATATTTCTTGAATCAGGCGCCTTAGACCGCTCGGCCAAACAACC AATTACTTGTTGAGAAATAGAGTATAATTATCCTATAAATATAACGTTTT TGAACACACATGAACAAGGAAGTACAGGACAATTGATTTTGAAGAGAATG TGGATTTTGATGTAATTGTTGGGATTCCATTTTTAATAAGGCAATAATAT TAGGTATGTAGATATACTAGAAGTTCTCCTCGACGCTCTCCCTTATGCGA CTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCG CCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCG GCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCC GAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGC CAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCG TAGAGGATCTACAACTCCACTTATTGTTAGGTAGAATTGTCCGTTAGTTG TTTATTAATTGCAATAATGGGGCGTCCAGTTTTGGCAACAGTGTCCTCTT ACCAGGACACCTATGAGTTTGCCTCATGGCAAACTAGAGGTGTTGAAAGT ATGCATGGTTATAATTAGAGCAATTCATTACCCTCTGAATCCTGCCGGTA TACCCCATTGTTCGTTATCTTTATTTTTGGCTAAAACCGCATTAAGAGCT TCGTTTACCGTCATGCAATGCGGTAGGTTATCGAAGTTTGATATCCCGCC AATATCAGGCGAACGCTTGTTCTTCAGGTAAGCATATTTCCGCGCAGCCG CCTCTACTTTCTGCTTGAACTCATGTTTTTGAGTGCGTTTTTTGGATAAC CGCAGATTGTCAGCCTTTGCTTTTGCCTTAGCGATCCATGAAGTCAATTT TTTGAGGCTGGTTGTTCCGGCACCGCCGGAAACTGATCTTTTTGTTTTTT TAACTTGTGACTTCTTATTCTTTATTGCCACGTCATCCTGACAGGGGGAG GGGGTATCATTTTGACATGGGGGTGTGGATAAAAAATTAAATAAAGCCAA TGTCTTAGCGAGAACAGCTTTAACCTTGGTTGCCGCTGAAGAGATCTTTA ATTTGCTTTCTATCAGCGCATTTTTGGCTTGTTGTGCGAAGGCCAAAAAG GATGGTGTAAACCGGTACAGGTTAGCGCGACGTTCACGGTGATCGCCGAT AACAATCTCTACAGACAGAATTCCTTTGTTTACAGCTTCACGGAATGCAC GAACGACGGTTGATTGGCTATAACCAGTTTCTGCCGCGATCAGGCGGTGA GGCTTGTGAATGAAGTATTCACTGGTTGTTGCCGCGAGATTTGCACATTG CGACAGGATATGCCCGGCGCTACGGGATAGACCGGAGTGTGTTACAAAGC AGGCCAATTCATAGCCAGAAAAAGTAAAATCGCTCATCGTTATACAGCTC AGGAAAGTGACTTTAGCCAGCATTACAATGCTGGTGGTTCTTACTACGTC TGTTAGCGCGTTGCCGCGACAGGTACCAGCACACCAGCATCAAGCAATCG CTTCATCAGCCACTGCTGACCTTTGCCGGTTATACGAGTCGTGAAAGAAA TCCTGCTTCCATTGCTTGTATCGATCACGGTTTCTTTAAGGGTGAAATAC CCACGAGATATGTATTCTTGTTTGGGGACGTTCCTGCGTTCACCGGTTGC GATCAGAATTCCGTTATCACGCAACCAGGTGAAGAGATAGTTTTGGCCCA GGCCGAGCACTTTGGCATAGTTGCCGATTAGAACCCCGCTGGCGGTAGCA ACGCGTTCAGCGAATTCGACTTTAGGTGCATCCATAAGCATTTTTTGCTC CAGCCGTTGCTTTTGCTCTGCCAGGTCGGCAGCCAAACGGAGAGCTTCAG GGAGACTCTGCGGAATAGCAGGTTGTAATCTTCCGGTTCGATAGTCGATA AATGTCTGGTTTACCTTCAGCCGAAACGCGGGAGAAATCCAGCCTGCGTA CTCCACAGCGAGCAATTCATGGGCAAAAGTGCCGCCGCCACGGCCTTCGA CCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTG TGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCAT AAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTG CGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGGTA GTCGATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAA GCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCC GACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCG GCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCA GAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCT CATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCG TCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAA GCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCG TGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAG AGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGA ACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAA AAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTT CCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGT GCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGA AGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCG CTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCA ACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCA CCCGTGGCCAGGACCCAACGCTGCCCGAGATGCGCCGCGTGCGGCTGCTG GAGATGGCGGACGCGATGGATATGTTCTGCCAAGGGTTGGTTTGCGCATT CACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTGAATC CGTTAGCGAGGTGCCGCCGGCTTCCATTCAGGTCGAGGTGGCCCGGCTCC ATGCACCGCGACGCAACGCGGGGAGGCAGACAAGGTATAGGGCGGCGCCT ACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAGGCGGCATAAATCGC CGTGACGATCAGCGGTCCAATGATCGAAGTTAGGCTGGTAAGAGCCGCGA GCGATCCTTGAAGCTGTCCCTGATGGTCGTCATCTACCTGCCTGGACAGC ATGGCCTGCAACGCGGGCATCCCGATGCCGCCGGAAGCGAGAAGAATCAT AATGGGGAAGGCCATCCAGCCTCGCGTCGCGAACGCCAGCAAGACGTAGC CCAGCGCGTCGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAA CGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGAT TCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGC GGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGT TGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCG CGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTC GACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGG TTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGAT GGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGA AACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTG ATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCC GGCCACGATGCGTCCGGCGTAGAG>pSacBII P1 cloning vector 16005bp GATCCTCTCCCTATAGTGAGTCGTATTAGCGGCCGCAAATTTATTAGAGCAATATAGTCC TACAATGTCAAGCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGT GGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCG TAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCG CGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCT TCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGG CGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCC CCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGT CCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCA GCCTAACTTCGATCATTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCA CATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGT TTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCG CTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATG CGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGC GGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGT TGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATAC GCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAA TGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCA TTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTG TATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATA CCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCC GTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCC CTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTA TCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGG CAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGC GTTTCGGTGATGACGGTGAAAACTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTG TCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGCTGTTCACGGGATGTTGGCGGG TGTCGGGGCGCAGCATGACCCAGTCACGTAGCGATACGGCAGTGTATACTGGCTTAACTA TGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATCGACTACCTGGCACGACAGGT TTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATT AGGCAACCCAAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAAC CGGTAACAATTTTCACACACGGGAACCAGCTATGACCCATGGATTTACGCCAAGCTTGCA ATGCCTGCCAGGTCCGAAAGGCCCTTGCCGGCGGCAACTTTTGCCCCAATGAAATTGCTT CGCTGTGGGAGTACGCAGGCTGGATTTCTCCCGCGTTTCGGGCTGAAAGCTAAACCAGAC ATTTATCGACTATCGAACCGGAAGATTACAACCTGCTATTCCGCAGAGTCTCCCTGAAGC TCTCCGTTTGGCTGCCGACCTGGCAGAGCAAAAGCAACGGCTGGAGCAAAAAATGCTTAT GGATGCACCTAAAGTCGAATTCGCTGAACGCGTTGCTACCGCCAGCGGGGTTCTAATCGG CAACTATGCCAAAGTGCTCGGCCTGGGCCAAAACTATCTCTTCACCTGGTTGCGTGATAA CGGAATTCTGATCGCAACCGGTGAACGCAGGAACGTCCCCAAACAAGAATACATATCTCG TGGGTATTTCACCCTTAAAGAAACCGTGATCGATACAAGCAATGGAAGCAGGATTTCTTT CACGACTCGTATAACCGGCAAAGGTCAGCAGTGGCTGATGAAGCGATTGCTTGATGCTGG TGTGCTGGTACCTGTCGCGGCAACGCGCTAACAGACGTAGTAAGAACCACCAGCATTGTA ATGCTGGCTAAAGTCACTTTCCTGAGCTGTATAACGATGAGCGATTTTACTTTTTCTGGC TATGAATTGGCCTGCTTTGTAACACACTCCGGTCTATCCCGTAGCGCCGGGCATATCCTG TCGCAATGTGCAAATCTCGCGGCAACAACCAGTGAATACTTCATTCACAAGCCTCACCGC CTGATCGCGGCAGAAACTGGTTATAGCCAATCAACCGTCGTTCGTGCATTCCGTGAAGCT GTAAACAAAGGAATTCTGTCTGTAGAGATTGTTATCGGCGATCACCGTGAACGTCGCGCT AACCTGTACCGGTTTACACCATCCTTTTTGGCCTTCGCACAACAAGCCAAAAATGCGCTG ATAGAAAGCAAATTAAAGATCTCTTCAGCGGCAACCAAGGTTAAAGCTGTTCTCGCTAAG ACATTGGCTTTATTTAATTTTTTATCCACACCCCCATGTCAAAATGATACCCCCTCCCCC TGTCAGGATGACGTGGCAATAAAGAATAAGAAGTCACAAGTTAAAAAAACAAAAAGATCA GTTTCCGGCGGTGCCGGAACAACCAGCCTCAAAAAATTGACTTCATGGATCGCTAAGGCA AAAGCAAAGGCTGACAATCTGCGGTTATCCAAAAAACGCACTCAAAAACATGAGTTCAAG CAGAAAGTAGAGGCGGCTGCGCGGAAATATGCTTACCTGAAGAACAAGCGTTCGCCTGAT ATTGGCGGGATATCAAACTTCGATAACCTACCGCATTGCATGACGGTAAACGAAGCTCTT AATGCGGTTTTAGCCAAAAATAAAGATAACGAACAATGGGGTATACCGGCAGGATTCAGA GGGTAATGAATTGCTCTAATTATAACCATGCATACTTTCAACACCTCTAGTTTGCCATGA GGCAAACTCATAGGTGTCCTGGTAAGAGGACACTGTTGCCAAAACTGGACGCCCCATTAT TGCAATTAATAAACAACTAACGGACAATTCTACCTAACAATAAGTGGAGTTGTAGATCCT CTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTA TATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTG TTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTT GCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTT CCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGGAGAACTTCTAGTATATCTACATAC CTAATATTATTGCCTTATTAAAAATGGAATCCCAACAATTACATCAAAATCCACATTCTC TTCAAAATCAATTGTCCTGTACTTCCTTGTTCATGTGTGTTCAAAAACGTTATATTTATA GGATAATTATACTCTATTTCTCAACAAGTAATTGGTTGTTTGGCCGAGCGGTCTAAGGCG CCTGATTCAAGAAATATCTTGACCGCAGTTAACTGTGGGAATACTCAGGTATCGTAAGAT GCAAGAGTTCGAATCTCTTAGCAACCATTATTTTTTTCCTCAACATAACGAGAACACACA GGGGCGCTATCGCACAGAATCAAATTCGATGACTGGAAATTTTTTGTTAATTTCAGAGGT CGCCTGACGCATATACCTTTTTCAACTGAAAAATTGGGAGAAAAAGGAAAGGTGAGAGCG CCGGAACCGGCTTTTCATATAGAATAGAGAAGCGTTCATGACTAAATGCTTGCATCACAA TACTTGAAGTTGACAATATTATTTAAGGACCTATTGTTTTTTCCAATAGGTGGTTAGCAA TCGTCTTACTTTCTAACTTTTCTTACCTTTTACATTTCAGCAATATATATATATATTTCA AGGATATACCATTCTAATGTCTGCCCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGT TGGTCAAGAAATCACAGCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTCGTTC CAATGTCAAGTTCGATTTCGAAAATCATTTAATTGGTGGTGCTGCTATCGACCTGCAGGG GGGGGGGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAA ATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTA TGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTCCAACATGGATG CTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCT ATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCG TTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTC TTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGA TCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTG TTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTT TTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGG TTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAG AAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCAC TTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCG GAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTC CTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAAT TGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAAC ACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGGCTTTGTTGAATAAATCGAACTTT TGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCCGTGGCAAA GCAAAAGTTCAAAATCACCAACTGGTCCACCTACAACAAAGCTCTCATCAACCGTGGCTC CCTCACTTTCTGGCTGGATGATGGGGCGATTCAGGCCTGGTATGAGTCAGCAACACCTTC TTCACGAGGCAGACCTCAGCGCCCCCCCCCCCCTGCAGGTCGATGCTACAGGTGTTCCAC TTCCAGATGAGGCGCTGGAAGCCTCCAAGAAGGCTGATGCCGTTTTGTTAGGTGCTGTGG GTGGTCCTAAATGGGGTACCGATGTAGTGAACATACGGAGTCTGGATATAGTCTTCGGCT GGCTGGAAGAAATCCTTCGGCAACTGTTTGAAGAAAGATGCTGCATCAGCACCAGCAAAC ATCCCCATCGCACCAGAAGATTTAACGCGCTCAATAATGTCGCGATATACAGTCTGGAAT TTGCCACGAATGATGGTTGCCCATACATCAAAGGACTGGTTAACCGGCAGAGCGATGTCA AAGGTGTCGGTCGCAAGAGTGCGCCAGATCATGATGCGAAGACGCAGCATATCCTGTTCA TGAGACAGGTATTCCTTCAGGGTGCGGAACTGTAGGGAACCCAGGTCCAGACCAAATTCA CGCTGTGCTTCATACGCCGCCTGTACCGTGTGCTCAGCCGCGATAACGAACTGACTTGGG AACAGGGTGTATTTCTTCATTTCGTGGTTGATCAGCGGGATCAGCTCAGGAGCGGCTTCA ATATTGATTTCCGTCTCAATTGCGATCTCAGTGCCTTTATCCGGCGCTTTGGAGAACGAC AGGGCAATCTGACCAATGTTGTAGTTCAGAGAGCAGGTAACAGTGATTTGCTCACCAGCA GCATTAGTAAACGAGTGAAGTAGGCTGCCGGAACCGTTATCAACAACAGACTTAATACGG TTAACGTAGATGTTAGTGCGACCTTTTCGGATTGGTACATTCTGGCCTTCGAAGTCTTCC ATCTTGAAGGTTGCGGTTTTGCTGGTGCCATCGGAGCTTGCCACCAGCACATAGCGGCGA CGTAACTGGCTGTACACACCGACGGATTGCATGTCCAGAACATCACCAGCAGCATAAGAA CCAAAAGATGAACCTGCCACGTTAAAGACTTCATAGATGTCGGACTGGTCACGCGTAACC GGAATGAAGGTACACGCATCAGCGGTAGCTGCCCCCAACTGAACAGGCAGGATCATCGCG AGGAATAAAGGCAGACGCATAACACCGTCAGAAACGCTCATCATCTCTGCTGCGACAGAT TCCAGCATCGCTTTATTAGTGGCATCCATGCTATTGCGGGTGGACTCTATCAGGCAGTTT TCCAGCGTCTGGTGGCAGGAGGCCAGAATTTCCGGACGCGGCATAGATTTATGTGCTGCG GCGTAGTCAGCCAGTGCACTTGCCCACGCTGTAGCGATTTGAGCGGTGGCATTATCAGAG ATACCCGCAAAAACCGGGTCTTTACGTGCAGCTTCAAGGATAGATGCGGCACGCGCGGCA TCATCTTTAATGAATTGGTTATCAGTACCGAACTGCGCAGTGCTTGCCCAGCCAAGCACA GCTTTAGAGCGTTTTGCGATATCTGCAATACGATTCTGGTATTCGCGTAAGTTACTCAAT TTACTCTTCCTTAAACACAAGGCACTTGTGTGAATCCCTTTTCGGAAGAGATTTTATTGA AAGTCACTTGTTGACTTTCTCGTGACAAGCAATTTTTTTATTTTTTTCGGGAGGAGGGGA GGAAGGTAAAATCCAAGGTGAAATCGTGGCGATTTCACCTTGAAATTTTAGAGTAATTTA CTTTAAAAACAGTCAGTTAATAGTGAAATTTGAATGGCGAAAGTTTAAGGCTTCGGCTTT TTATCGAGGCTCTTTCTAAGGATATGCCCAATCATCCTGTCGAGTTCTTCCTGTAACTCT TTTGAGAGTCGATTAAACTCATAAGAAAATGCACGGCCTTTCACGCGCTTCCTTGCAAAG CGATCCTTGTCCTCAAATTTCCATAATTCAGTAACTACGGACTTATCTTTAGAACCTTTA TCCGTGAGTAGTGAGGCTTCCTTTGTTATCAAGCGCAGGATTTTATTTTTAACTTCATCT TCGGCCATTTCTTCAATGGATAAGATGTCGTTTATTTCCGGGGATATGTTTTGAATAAGC TGATCAAACTCTAAATTCTTGTTCCCCATTTCGTCGCCAACAGCACAAAGCGTTTTGTAG TCCGAAAAGGTTAATTCCGACTGCACAGGGAAAAGGGCGACTAATTCTTCCGGAGCACTC GCTGCCTGGAGAGCACGCGTGACCTTCGCCTGAGACAGCCCTTCTTTGGCTGCAATATCC TTCTGACTCATCCCATCATTTTTCATTCGCATCAAACGCAGACCTATTTCTCGAATGCTG TGCTGCAATGCTGTCTGAACGTCTTTCGCTAAATTTTGCGCTTCCTGAACGCTGATCTCC TGGTCCGTGACTAAAACCCGCAACCCTACGTTCTCTAAGATGGCAGAAGCTCGACGCCGG GAACCATCCAAAATTTCAATTTTCCCTGTAGCCCGTCTAACACCTATTGCAGGGTAAAAT TGCTGATGCTTAATAGTGCTTCGGATACTTTTTAATGATTTTGGCGTAAGAGATGCCTGG TCACGCCCGTTGTTATGCTGATCAACAAAGGTATCGCTTTCTACCTGGTTCGGAGGTATT ACCTCTTCAATAAATGTGGCCTGGCGACCAGTTGATAACTTGAATACCTGCTCGACTCGA TCGCCAGAGGCTGAAGAACTATCAAATCCGCTTAATATTGAAGGATTAAGGGTTCGCCCA ATTGTTGGTCTGTTTTTCTTTGACATGGGGGTTTCTTACTCCTCAGTTAGATCTGATAAA TTCAATACGGTCAAAAACTGCTTTAGCAAAATCTTCCGCGGCAATTCGCGCGTTCTTCAA TGCATCAGCACTACCAACATACGTTGCCGGGTTAGCTGAAATAACAGTGTCAAAAGACTC GCCGCAGCGTTCAAAACCGTCAAGGCGAGGGAGGAAGACATCAAGCATATCCCCACCGAA CACTTCTTTAGCCAGGCTATGGCAATACTTATGGTCTGCCTTGTTACTCAACTTGGACAT AAAACCAATGTTAGTCGCAAGCTGGCACTCGCAGCCTTCATCCGAAATGAGTTTCACCAA CTCAGGAAGGCGGGCAACGTATTTAAGCGATGAGTGGAAATCGACAGTTGCTGGCGGCAG AGGTGTAAACAGTATATTGGCCGAGGCCAAAGCATTTTTCAGGAAGGCGTCAAGGTGAGG ACCACTATCAACGAGGATAAAGTCATAATCGCTCTTCAGCTTATCAATCACATTTTCTTT CAGGACAGCATGGATGTTCTGACCCGGTAGATGCTCATTGCACAGCTCTCTCCAATCGGA TGCAATAAAGGCATCGTCAATCGACGCAGGCATAACGTCAACCCCAGGTACAACAGAAGG AACAATAAACTCCTCTAACAGCTCTTCACGGCTTACATTCTGCAACATAGCCTGTGCAGA TGTTGCGTTTACGATACCAATAGAGTGTTTATGGCTTAAAAACATCGTTGCTGAAGATTG CGGATCAAGGTCAATAACCAGAATCCTTAAATCCTCCATAAGAAGATGAGGGTGAGCACG CATTGCATGCGCCAGAGAAACCGTCGATACAGTTTTTGACACACCGCCTTTAAGATTGGA GATGAAAATCACATACGCTTCGCTGTAGCGATCCCGGTATTTTGGCACTCCGCGATGTTC ATATATGTCAATGATGTTCTGAATTGACATCGCATATTTCATTGAAGAGCCAGCAGGGCG TTTATCGAAAACATAACCCTTTTCTTCCATTTCACTTACGGCATAGTCAACGTTCGCTCG AGTCAGTAGAGGCAATTTTGCCAGTGCCGCTTTCGCATAGACCTGGTAAAACTCGTTCGC GTGTAGCTCATCCTTTTGCAACTGTACTTGTTCAGTCAGAACATTGAGCATTCTGTTTGC TCTTTGAGCAACCTTGTGAAGCTGGCTGGAATCACTCATCGAAAGTCATCCTTTATGCTG TATTTTTGAATTTAATTAAAAATGCTGCATAAAATAATAATGTATGCGTAGATGCTTGTA CATAGCATTCTCTGCATGCTTACTCCTTTGCCAAGTGTGTCACAACATGCTTTGGCATCC TCCACGCATAAAGGACGCGGATTTCTTCTACGTTCAGGTTGTAGCCTGAATCATTTTGGT GATTTCCTGCTTCAATGGGCAGCCAAGCTTCACTCTCTATCAACTGGTGAGAACGGCTTG CCCGGCAGCTTCGATGTGTGCTGGAGGGAGAATAAAAGTCTAAGATGTGCGATAGAGGGA AGTCGCATTGAATTATGTGCTGTGGAGGGATCGCTGGTATCAAATATGTGTGCTGGAGGG AAAGGCAGAGAATTACATGTGCACTGGAGGGAAAAGCGGATGGACAGATGTGTGCTGGAG GGAAAGTCTGGGCAAACTGAGGGGCGTCCCCCTCCAGCGCACATCAAAAACAGGAAATTG GACAAGCCTTCCCGGCAGCACACATCTTTTTAATGCGGTTTCCCTCCAGCACACACTTAT TCGGGGAGTTTCAGCTTTGGATTGCGAGAATGGACGATTACAAAACTTTCCCGGCCTTTC TTCTCAATAGAACAGTCGAGATAGCCGATTGTTTTAAGCTGTTCTATCGCTTTCTTAATG ATACGGTTTTGCTCGCCAACAGCTGACTGCAAAGCCAGGCGCTCACGGATTCGCGCGAAC GATAGCGGCAACGGGTTCTGCGGAAGGCTTTCGATGAAAGTGTAAATGGCTTGTGCAGCT TCTTTCTTCGGAAGGGCACGCAAGGCGTGGTGTTGCAACAGAACGCGATAATCAAGCTGG AACAGCTCCCACAACTTCGAATCAGCCTCCAGCTCTATCAGATCAAGGTCAGCATCAAAA CGCCCGACCTTCAGCAGACCAGTCTGATAGCCGCCTTTAGCATCTTTTCCGCGCTTAAAA GCGATACCCTTGTTACGCAAGCGTCCAAGTGATTCATGAATGGTTAAACGCAGTTTCGCA TCCAGACGTTTTGAGGGGAAACCACAGGCTTTAGCGAATTCCTGAAACGATAACTGGATG GTGTTTGAGGACAAGCCGTATTTGCTGAACGCGTAGATGACACCGATCCACGTTTTGAAA TCAGTATCCATATCGAGTCGAGGACCGGTGATTTTAATATCATCGTAACCCTCGGCTTTA GCTATCTCCAGCTGGGAAAACGCTTTGGTGGCATCAATCTCTTTACTTTCTCCTTTGCTC TTTGATGGCTTCGGCACGAATACCCCCAAGCGCATCAACGCTACAGGCTGCACAGTGTTG TTTGAATTAACTGTTAGTTCTTTTGCCTTACTTTCAATGTCTGCGTAAAGAATATCGGAG ATAAATGATTGATTCATATTACTTTTTCCGAATTGTGTGGATAGTTTTTATAAGTGGTGA TAACTACCCAAGCTTTCCCGTCAACACACATCCTATATCCCGCCAGCACACATTAGCAAC CCGTCAGCACACATTTTTATCCCTCCAGCACACATCGTTTTCCCTCCAGCACACATCGCG ATACACTTCTAAGCCAGACGTGGCGCGGCCTGCAACGATCAGGGATCTATATGGATCTAA TTGGGATCTGTATGGACCTGATTATTGGATCTATCCAGTGGATAATGTGGATAAGTGAAA AACCGGCCAACGTAGCCGGTTGGAAGGGAGTCGTATTATTCTACGCTTTCGATGAGAAGA CCATGTTCATAGCATTTAAGCTCATCGCCCTCGTACAGGAATTGGTATCCAATACCACCA TATTCAGGCACATTAGGGAATAACTCATCACTTACCGAAGAACAAATCACACCAATGCAG CGATCAACGCCTTCTCGTTCTTCAGTGCTGAAAAAATCCTCTTCGGTAAGAACATGAGTA CATTGCTCATCAGCATAGGTCGGAAATACATGCTCAATGCAATCCGGGTGTTTTAAACCA AGCTGATCGGCAAGCTCGAAAGCACGACGGTATTGTTCAGATCCTGGCTTGCCAACAGTG ATTTGCTCAATTTTGTAGATTGAAGTCGCTTTGTTGATAGTTTGCTTTACTGTTACTTTA TCAGACATAAAAATCCCTTTTAGTTACCGCTAATAGCTCGGTTGTAATCATTAACGTTGC GATTCTTCCTGTTAATCCCCATCAGCATCGTTTCTGTATCGAGGATATACGCTGGCAGAT CATCAAAATATTCACTGCTAAACTCTGGCATCCTACACATAAATGCACTTTTGGGGGCAG GGTGGTTAACCTTTGTCGGCGTCGGCGTTAAATTCGCTGATCGACTCCCGGAGCAACCGC TGAGTGTCAGCAGGAATACGCTGGCGAACATTACCCGCCGCAACCAGTTGTTTCTGAACT TCAGCTTTTCGTTCCATTTGCCTGTCAGCATACTTGGCTTGTTCTGATTCATTTTTCACT TCCTGGCTGTGAAAATGTTGCTCTGCTTTGTTCATCGTCTCAATGGTCTGGTTAAGATCC ATTATTGACTTATCACGTTCCTTAACAGCCTGATCAAGACTGCCAATTTTCTCTATGGCT TGCTTTAGCTGATGACGTTCCCATGCAAACCCAGCACCAACAAGTGCGCAAATCAGAACA AGAACACCAGTAGCAGCAAGTTTCTCCTTCAAAGACAAAGCTGTTTTTAACGTAGAAAAG AATGACATGTCTTCCTCCTGAAGAAAAATTATCAATGAAGTCCTTTGTTACTGTGCCGCT TTGTTTAATTCATCAAGAACAGAATCAGGAACCAAAGCGGCAACTGCGCTGGCTGTGCTG GCCTTATTTGCTGATGCTTCCGCAAGCGCGGTACCGGTAGTGTTAGACCTGAACAAGGTT TACTAAAAATCCGTAAAGAACTTCAATTGTACGCCAACTTAAGACCATGTAACTTTGCAT CCGACTCTCTTTTAGACTTATCTCCAATCAAGCCACAATTTGCTAAAGGTACTGACTTCG TTGTTGTCAGAGAATTAGTGGGAGGTATTTACTTTGGTAAGAGAAAGGAAGACGATGGTG ATGGTGTCGCTTGGGATAGTGAACAATACACCGTTCCAGAAGTGCAAAGAATCACAAGAA TGGCCGCTTTCATGGCCCTACAACATGAGCCACCATTGCCTATTTGGTCCTTGGATAAAG CTAATGTTTTGGCCTCTTCAAGATTATGGAGAAAAACTGTGGAGGAAACCATCAAGAACG AATTCCCTACATTGAAGGTTCAACATCAATTGATTGATTCTGCCGCCATGATCCTAGTTA AGAACCCAACCCACCTAAATGGTATTATAATCACCAGCAACATGTTTGGTGATATCATCT CCGATGAAGCCTCCGTTATCCCAGGTTCCTTGGGTTTGTTGCCATCTGCGTCCTTGGCCT CTTTGCCAGACAAGAACACCGCATTTGGTTTGTACGAACCATGCCACGGTTCTGCTCCAG ATTTGCCAAAGAATAAGGTCAACCCTATCGCCACTATCTTGTCTGCTGCAATGATGTTGA AATTGTCATTGAACTTGCCTGAAGAAGGTAAGGCCATTGAAGATGCAGTTAAAAAGGTTT TGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTG ATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAAAAAGATTCTCTTTTTTTATGAT ATTTGTACATAAACTTTATAAATGAAATTCATAATAGAAACGACACGAAATTACAAAATG GAATATGTTCATAGGGTAGACGAAACTATATACGCAATCTACATACATTTATCAAGAAGG AGAAAAAGGAGGATGTAAAGGAATACAGGTAAGCAAATTGATACTAATGGCTCAACGTGA TAAGGAAAAAGAATTGCACTTTAACATTAATATTGACAAGGAGGAGGGCACCACACAAAA AGTTAGGTGTAACAGAAAATCATGAAACTATGATTCCTAATTTATATATTGGAGGATTTT CTCTAAAAAAAAAAAATACAACAAATAAAAAACACTCAATGACCTGACCATTTGATGGAG TTTAAGTCAATACCTTCTTGAACCATTTCCCATAATGGTGAAAGTTCCCTCAAGAATTTT ACTCTGTCAGAAACGGCCTTAACGACGTAGTCGAGGGACCTAATAACTTCGTATAGCATA CATTATACGAAGTTATATTAAGGGTTCCGGATCAATTCTCATGTTTGACAGCTTATCATC GATAAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGT ATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGC ATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCGTCCATTCCGACAGC ATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCA CCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTA CTTGGAGCCACTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATCTACCCA CTAGTCAATTCACCATCTTGTTGAGAAATAAAAGAAAATGCCAATGAAGTATCGGCATTT TCTTTTTGCTGTTATTAGTTGACTGTCAGCTGTCCTTGCTCCAGGATGCTGTTTTTGACA ACGGATGTTTTATTGCCTTTGATGTTCATTAAGAAGCTTGGCGCAAATGTTGCCTTTTTA TCCTCGAAGAAGCCTCTGTTTGTCATGTAGCTTGTGATAACCACATTGTTGCCTTTGGCT TGCGGCACTGCGAAGTGAGAGTAAGTGAATGTCACATCGTTTGGATCAAGACCCATTTGC AGCACAAGCCCTGTTTTGTTCAGCGGCTTGTAAGGGCCGGTTAAAGAGTTTGATACATAA CCAAGCATGTAAATATCGTTTGAGTTAATACCATCGATCGTCATTTTTGAACCGCGTGAA TCAGTGAACAAGTACCATTTGCCGTTCATTTTGAAAACATTCGCGCGCTCGATTTCATCA GTTACCGTGTTTGAAGTGATCAGCGGCTTCATTACTTTTTTCAATGTGTAATCATTATTT AACTCTATGATACCGAGGGCGCCGTTCGCTAACTCAGCATCGCGTTTTTTAGCGCTCTGC TGAAGCTTCTGGCTTTCTTTACGGAAGAAGTTCGTGCCGCCGCCGTAGTACGCTTTGTTA AATAAAGATTCTTCGCCTTGGTATCCGTTTTCTGTTCCCGTGTTGGCTTCGAATACAAGG TATTTATGGCCTTTGTCTTCAACGTAGTGAGGGTCTCTCAGCGTATGGTTGTCGCCGGAT GTATAATTGCCTTCATCGATAAACTGCTGAACGTTCTGATATGTTTTTCCGTCTCCGTCA AAAATCGTTTTGTGATCTTCCACTCCGTTGATTTTGAGTGTGTCATCAGATTTTGACACA TTTACCTGCGCTGTTGTCAGGCTTTGTTTGCCGTAATGTTTACCGGAATAGTCAGTGTAG AATAAACGGATTTTTCCGTCAGATGTAAAGGTTGCAGAACCGGACCATTCTTGCGTCTGA TCTTTCAGGATCGGATCGTTGGCGTCGAACTTATCGCTGTCTTTAAAGACACGGCCCGCG TTTTTCCAGCTGTCGATTGAGTTGTCGCCGACCTTTTGATAAAACATGTAGATTGATGTG TCATCAGCGTCTTTCGGGCTTCCCGCAAGAGCAAACACAACGTGATAGCCGTTGTATTCA GCTACTGTTCCGTCAGCGTTTTGCAGCGGCCAGCTGTCCCACACATCAAGTCCTTTTGCA GACTCAATATTTTTAATCGTTGATTGATCGAATTGAGGCACTTGGTATTTTTCGTTTTGC TGCTGTTTAGGGATCTGCAGCATATCATGGCGTGTAATATGAGAGACGCCGTACGTTTCT TTGTATGCTTTTTGGTTATTTTCTTTCGCGAAGGCTTGAGTCGCTCCTCCTGCCAGAAGT GCAGTCGTAAAAGTCAGAACTGTGGCTTGTTTTACAATTTTTTTGATGTTCATGTTCATG TCTCCTTCTGTATGTACTGTTTTTGCGATCTGCCGTTTCGATCCTCCCGAATTGACTAGT GGGTAGGCCTAATTGGCCGTCGACATTTAGGTGACACTATAGAAG | |||||||||||
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