BDGP Resources
pOT2 Vector
The SP6 primer does not work well for sequencing, so we use the PM001 primer for 3' EST sequencing, and the T7 primer for 5' EST sequencing.
T7 primer: AAT ACG ACT CAC TAT AGG
PM001 primer: CGT TAG AAC GCG GCT ACA AT
>gb||pOT2 pOT2 vector ORGANISM pOT2 vector) CGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATA CGATTTAGGTGACACTATAGAACTCGAGCAGCTGAAGCTCCAATGTGATG GTCTAGAGGATCCGAATTCCCAGCACAGTGGCGATGATATCAGATCTGCC GGTCTCCCTATAGTGAGTCGTATTAATTTCGATAAGCCAGGTTAACCTGC ATTAATGAATCGGCTGCAGTACCCGGGAATTTAACCCGCCTAATGAGCGG GCTTTTTTTTGTGATCCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGC GTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTG TTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCA GCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGC CACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAAT CCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGT TGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACG GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACT GAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGA GAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGC ACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGG GTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGG GGCGGAGCCTATGGAAAAACGCCAGCAACGCGGATCACAACAAAAAGCCC GCTCATTAGGCGGGCTAAATTCTCATGTTTGACAGCTTATCATCGATAAG CTTTAATGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGA AAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAA GAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGAC CGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGC ACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCT CATCCGGAGTTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGA TAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTT CATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATA TATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAA AGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTT TCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCC GTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCC GCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCA GAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCG TAATTGGTACGTCGAEcoRI adapter sequence:
5'-GAATTCGGCACGAGG-3'