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Materials: BAC Clones

BACPAC Resource Center in the Dept. of Human Genetics at the Children's Hospital Oakland Research Institiute

The BDGP Drosophila melanogaster BAC library was prepared by Kazutoyo Osoegawa and Aaron Mammoser in Pieter de Jong's laboratory at Children's Hospital Oakland Research Institiute. The library is named "RPCI-98" and was constructed by partial EcoRI digestion of Drosophila DNA provided by the BDGP from the isogenic strain y[1]; cn bw sp, the same strain used for the P1 and EST libraries. A page describing the library and how to order individual BAC clones, the entire library, or filters for hybridization from the BACPAC Resource Center can be found at

http://bacpac.chori.org/dromel98.htm

Please note that the BDGP and BACPAC Resource Center have slightly different nomenclature systems for the BAC clones. A clone called "BACR48M07" by the BDGP should be referred to as "RP98-48M7" in communications with BACPAC.

In both systems, the first number refers to a library microtiter plate, and the letter and second number refer to the row and column in a 384-well plate format. The BDGP names are "zero-padded". In the BACPAC nomenclature, based on a format suggested by the NCBI, the constructor is abreviated to "RP" and the library is separated from the constructor by no "-" character. Instead the "-" character separates the library from the clone coordinate and the clone coordinate follows the library name without any space characters. We apologize for any confusion arising from the two parallel naming conventions; they exist because we wanted BDGP clone names (BACR for EcoRI) to follow the nomenclature already established for two new BAC libraries produced by the European Drosophila Genome Project, BACH (HinDIII) and BACN (NdeI), in cloning vector pBeloBAC11. BACN is cloned into the BamH1 site."

This page last updated on: 4/22/08
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