Non-BDGP Fly EST Projects


The Exelixis ESTs were submitted by the BDGP on behalf of Exelixis. The cDNA libraries were NOT made from the y; cn bw sp strain used for genomic sequencing. Below is a description of each library.

Unfortunately, the Exelixis cDNA clones are not available for distribution.

Oliver Lab

A testis Drosophila cDNA library was constructed by Justen Andrews in Brian Oliver's laboratory at the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) which is part of the National Institutes of Health (NIH) at Bethesda, MD, USA. Poly (A) + RNA was prepared from dissected testes from 1-5 day old y w [67cl] Drosophila melanogaster flies, after removal of the paragonia and inclusion of the ejaculatory ducts and seminal vesicles. The size fractionated cDNA (1-6kb) was directionally cloned in the Stratagene Uni-Zap XR vector using EcoRI and XhoI restriction sites. pBluescript SK (-) is produced after excision. The host strain is E.coli SOLR.

This library has been used to generate 7297 ESTs that can be queried at dbEST at NCBI. Array data using these cDNAs is available at the NCBI Gene Expression Omnibus (GEO) site.

The Geneservice in the UK has approximately 3930 clones available from plates (labelled bs) numbered 3-24, 26-37, 40-42, 44-46 and 49. All clones and plates except bs49 are available on one double-spotted high density filter for screening by hybridization from them.